Use of the Architect-i2000 estradiol immunoassay during in vitro fertilization.

reported by other researchers using enzymatic methods, e.g., a within-run CV (n ϭ 11) of 4.4% at 80 ␮mol/L (10) and a between-run CV (n ϭ 12) of 10.4% at 75 ␮mol/L (7). Analytical recovery was examined using plasma specimens (160 ␮L each) from a reference pool containing 30.6 ␮mol/L mannose. Samples were supplemented with 40 ␮L of water, 500 ␮mol/L mannose, or 1 mmol/L man-nose. Each supplementation was performed in triplicate and yielded recoveries of 104.0% for the lower-concentration mannose samples and 102.6% for the higher-concentration samples. We studied potential interference from endogenous substances using a plasma pool (mannose concentration, 79 ␮mol/L) obtained from healthy volunteers. A one-tenth volume of the solution containing a possible inter-ferent was added to the plasma. Glucose at 50 mmol/L, fructose at 200 ␮mol/L, free plus conjugated bilirubin at 340 ␮mol/L, hemoglobin at 5.0 g/L, uric acid at 600 ␮mol/L, and ascorbic acid at 20 mmol/L did not affect the measured mannose values. The mean plasma mannose concentration of 15 samples from healthy individuals (10 from females and 5 from males), collected in the morning after fasting, was 35.6 Ϯ 12.6 ␮mol/L. The mean glucose concentration of the same plasma samples was 4.64 Ϯ 0.87 mmol/L. The observed mannose value was comparable to those reported by other investigators: e. Both mannose and glucose concentrations in plasma samples (n ϭ 21) from nondiabetic and diabetic patients were measured. A close positive correlation (r ϭ 0.779; P Ͻ0.01) was observed between mannose and glucose concentrations. Our data are consistent with those reported previously (6, 7). We also measured the mannose and glucose concentrations in plasma samples collected at 0, 30, 60, and 120 min after oral administration of 75 g of glucose to healthy, 14-h fasted volunteers (two females and three males). The plasma mannose concentration gradually decreased until at least 120 min after glucose ingestion, whereas the glucose concentration increased transiently, as is widely known (Table 1). To the best of our knowledge, this is the first report of the time course of changes in the plasma mannose concentration under physiologic conditions. We are now studying the mechanism of the change in the plasma mannose concentration induced by glucose load. Simultaneous determination of arabitol and mannose by gas-liquid chromatography in experimental candi-diasis. Relationships between glucose and mannose during late gestation in normal pregnancy and pregnancy complicated by diabetes mellitus: concurrent concentrations in maternal plasma and …